Multipletelophase arrest bypassed (tab)mutants alleviate the essential requirement for Cdc15 in exit from mitosis inS. cerevisiae

The Mitotic Exit Network (MEN) proteins – including the protein kinase Cdc15 and the protein phosphatase Cdc14 – are essential for exit from mitosis inSaccharomyces cerevisiae.To identify downstream targets of the MEN, we soughttelophase arrest bypassed (tab)mutations that bypassed the essential requirement forCDC15.Previous studies identifiednet1tab2-1andCDC14TAB6-1as mutations in the RENT complex subunits Net1 and Cdc14, respectively, and revealed that the MEN acts by promoting release of Cdc14 from its nucleolar Net1 anchor during anaphase. However, the remainingtabmutants were not characterized.