Patent AT-E400655-T1: [Translated] POLYNUCLEOTIDE SYNTHESIS USING PROCESSING ENZYME

The present invention pertains to a method for polynucleotide synthesis, comprising the steps of: (i) reacting a polynucleotide processive enzyme, e.g., a polymerase or TdT, with a nucleotide substrate under conditions suitable for enzyme activity; and (ii) modulating the conformation of the enzyme, e.g. using radiation, to allow incorporation of a predetermined nucleotide.