Patent AT-E401396-T1: [Translated] METHOD FOR FUSING DENDRITIC CELLS WITH TUMOR CELLS AND MEDIA FOR SUCH METHOD

Fusing dendritic cells (DC) and tumor cells (TC) in which the two cell types are mixed; optionally washed; centrifuged, then the pellet taken up in electrofusion medium (EFM) and subjected to electrofusion. EFM contains, in pyrogen-free, sterile, distilled water 20-150 mM sugar (I), 0.05-1 mM magnesium salt (II) and 0.01-1 mM calcium salt (III). Independent claims are also included for the following: (1) an Electrofusion Medium (EFM); and (2) a washing medium (WM) containing 270-310 mM (I), 0.05-1 mM (II) and 0.01-1 mM (III).