Datasets

3 datasets

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HealthData.Gov

Retrovirus-delivered siRNA

The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high production costs…

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Updated Oct 29, 2026

HealthData.Gov

Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant

We are developing retroviral-mediated gene transfer to human fibroblast-like synovial cells (FLS) as one approach to characterizing genetic pathways involved in synoviocyte pathophysiology. Prior work has suggested that…

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Updated Oct 29, 2026

HealthData.Gov

Retroviral vectors for establishing tetracycline-regulated gene expression in an otherwise recalcitrant cell line

Background Tetracycline-regulated systems have been used to control the expression of heterologous genes in such diverse organisms as yeast, plants, flies and mice. Adaptation of this prokaryotic regulatory system…

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Updated Oct 29, 2026